Benefits of MATCHmAbTM
Peptide Mapping
- Fast service – quickest turnaround time of any peptide mapping service
- Fast answers – scientists available to directly answer questions within hours
- Coverage – industry leading coverage over whole protein sequence using our unique protein sequencing technology
- Quality – years of experience and well defined protocol
- Top equipment – Thermo Orbitrap and Bruker TIMS-TOF mass spectrometers
- Software – access to our interactive coverage viewer to explore the data
Beyond Binding Assays: Specificity Plus Reproducibility.
Since the protein sequence determines structure and function, rapid confirmation of the protein sequence offers an ideal complement to performance validation assays when revalidating antibodies. Our peptide mapping mass spec service offers a precise, repeatable, and full confirmation of the protein sample and sequence information. This ensures the antibody is as expected, reducing the need for revalidation or troubleshooting downstream.
Reproducibility Impacts.
Reproducibility impacts.
“We got very quick answers that confirmed the sequence of 4 samples and saved us time on troubleshooting.”
– Max London, Research Scientist, Phenomic AI
Who Uses MATCHmAb™ Peptide Mapping.
Researchers, Antibody Users
Researchers use tool antibodies every day, across many applications. Sequence confirmation is the first step in troubleshooting, or daily quality checks to prevent issues.
Antibody Vendors, Producers
Easily verify each new lot. Offer premium Verified by MATCHmAb™ products, or an add-on service.
Antibody Engineering
Cell line instability could introduce mutations to humanized antibodies. Verify the CDRs have not changed or confirm the full expressed sequence matches the engineered one.
CRO, Custom Developers
Checkpoints to increase confidence. Avoid troubleshooting re-runs with clients.
Who uses MATCHmAb™.
Researchers, Antibody Users
Researchers use tool antibodies every day, across many applications. Sequence confirmation is the first step in troubleshooting, or daily quality checks to prevent issues.
Antibody Venders, Producers
Easily verify each new lot. Offer premium Verified by MATCHmAb™ products, or an add-on service.
Antibody Engineering
Cell line instability could introduce mutations to humanized antibodies. Verify the CDRs have not changed or confirm the full expressed sequence matches the engineered one.
CRO, Custom Developers
Checkpoints to increase confidence. Avoid troubleshooting re-runs with clients.
How It Works: The Evolution of Peptide Mapping & Analysis.
| MATCHmAb™ Enhanced Peptide Mapping | MATCHmAb™ Rapid Peptide Mapping | |
|---|---|---|
| Purpose | Consistent and high-quality, near 100% coverage. | Fast and low-cost sequence confirmation for routine checks, first step troubleshooting. |
| Workflow | Typically trypsin/chymotrypsin digest, followed by LC-MS, and manual data analysis to pick out the signal peaks. | Typically pepsin digest, followed by LC-MS/MS and automated data analysis. |
| Speed | 1-2 weeks | 4-5 days |
| Throughput | Low | High |
| Coverage | As close to 100% as possible | Approximately 95%, not guaranteed |
| Report | Chromatogram, Peptide Map, coverage report if selected | Sequence confirmation and coverage viewer |
| MATCHmAb™ | |
|---|---|
| Workflow | LC-MS/MS, single or dual digest |
| Bioinformatics | Real-time analysis, purpose built algorithms |
| Speed | Fast: 1-4 days |
| Throughput | High: > 100 per week |
| Coverage | Higher, and amino acid level |
| Traditional Peptide Mapping | |
|---|---|
| Workflow | LC-MS only, single enzyme only |
| Bioinformatics | Post-processing, off the shelf tools |
| Speed | Slow: weeks |
| Throughput | Low |
| Coverage | Lower, and only peptide level |
Peptide Mapping Price.
MATCHmAb Enhanced Peptide Mapping
- Price may vary depending on your specific analysis goals.
MATCHmAb Rapid Peptide Mapping
- Please inquire about volume discounts and upgrades.
Getting Started is Easy.
Here’s what we need:
- 20 μg of each protein of interest
- Reference sequence(s) in FASTA
- The sample should be reasonably pure and not a polyclonal mixture.
Don’t have the sequence yet?
Other Antibody Characterization Services.
Rapid PTM Analysis
Locate, identify and quantify post-translational modifications. Compare between samples.
Sequence Variant Assessment
Identify and quantify variants of your intended antibody within your sample, and consider other contaminants.
Glycosylation Analysis
Find glycosylation site occupancy, and profile their relative abundance %. Compare many samples.
Peptide Mapping
Match the masses of individual peptides to the expected masses from a given sequence, maximizing sequence coverage and confidence.
Disulfide Bond Analyses
Analyse peptides cross linked by disulfide bonds. Quantify disulfide bond shuffling in engineered constructs.
Intact Mass, Custom Analyses
Please inquire about intact mass analysis, subunit analysis, etc.
Talk to Our Scientists.
We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.
Antibody Sequencing, Discovery and Proteomics Services.
Antibody Sequencing & Discovery Services.
REmAb®
Monoclonal antibody sequencing from small protein samples, no need for hybridoma or DNA information. Full sequence in record time.
Explore REmAb
REpAb®
Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
Explore REpAb
MATCHmAb™
Antibody verification by real-time peptide mapping. Antibody sequence confirmation service for reproducibility.
Explore MATCHmAb
HDX-MS
Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore Epitope Mapping
SPR Analysis
Label-free evaluation of antibody-antigen binding relies on a Surface Plasmon Resonance technology. Combined with our expertise in antibody interactions, this results in high sensitivity and the lowest noise even in complex analytes.
Explore SPR Analysis
NovorIg™
Quantify the immune response by accurately profiling the vast antibody-protein repertoire and its different isotypes, and subtypes in serum over time.
Explore Immune Response Profiling
We Have Sequenced 4000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics