Abstract

In this on-demand webinar, we discuss why it is important to characterize antibodies based on their physical properties not just by what they bind, and how you can easily do the former via mass spectrometry-based protein sequencing.

Key Takeaways

  • Antibodies are generally characterized based on what they bind, not their physical properties, limiting researchers’ ability to find and keep good antibodies
    $800M is wasted yearly on antibodies that either don’t bind or bind the wrong target (Bradbury & Plückthun, 2015)
  • Hybridoma sequencing is a great place to start, but ~30% of antibodies have additional chains which cannot be identified just through DNA sequencing
  • In taking an MS/MS approach you can characterize and immortalize your best antibodies from the protein itself, while identifying additional chains and relevant post-translational modifications