Surface Plasmon Resonance Service.
RapidSPR™ is our Surface Plasmon Resonance (SPR) service, which can determine the on-rate (Kon), off-rate (Koff), dissociation constant (Kd), and antibody affinity (KD) for antibody-antigen interactions. We offer detailed antibody kinetic profiling and SPR assay development, antibody screening SPR and epitope binning SPR. Our expertise is Antibody-Antigen Interaction & Binding Kinetics Analysis.
How SPR Analysis Works.
Our method for label free evaluation of antibody binding and biomolecular interaction relies on a Surface Plasmon Resonance analysis technology from Biacore and Nicoya. This nanotechnology-based SPR platform enables immobilization of binding partners in both orientations on the sensor chip with low levels of bulk shift, high sensitivity and low noise. Our Biacore platform allows for higher throughput SPR services with excellent results.
| SPR | BLI | MST | ELISA | |
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| Label-free |  |
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| High-sensitivity (low pM) | |
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| Real time, continuous flow | |
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| Full Kinetic Analysis | |
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| Highest precision | |
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| Highest reproducibility | |
The Rapid SPR Service Advantage.
Unmatched SPR Accuracy and Reproducibility.
Kinetic analysis and validation is performed by our PhD experts, in triplicate, to meet the highest accuracy standards in the industry. We analyze residuals for validation of fit curve accuracy, and conduct advanced biophysical analysis. Kinetic parameters are reported with standard deviation of the triplicate set (and not just a standard error of a single run).
Surface Plasmon Resonance on Biacore 1k.
Our latest Surface Plasmon Resonance Biacore instrument is the Biacore 1k, which offers the perfect balance between high-throughput SPR analysis and versatility.
Complex SPR Assays and Difficult Samples.
We work with antibodies and antibody fragments (Fab, scFv), peptide antigens, and large complex protein antigens. With our expertise we can work with novel projects that don’t fit standard workflows.
Expert Analysis and Deeper Insights.
Intense analysis means optimization of binding conditions for every interaction are performed individually for increased accuracy and optimal signal/noise ratio. We provide deeper insights into each interaction with our full regeneration screen using different conditions (acidic, basic, chaotropic and high salt).
Applications of SPR Analysis
While there are many possible applications of surface plasmon resonance analysis, Rapid Novor is highly focused on antibody research and development:
SPR antibody binding affinity measurements
Off-rate ranking analysis
Cross-reactivity and specificity studies
Drug Discovery, antibody developability
Peptide library screening
Custom assay development
Success Stories.
“The sequences have allowed us to have an “insurance policy” of sorts for critical proteins used in our various products.”
“We’re focused on coupling cytokines with a highly engineered VHVL of an antibody. So by sequencing the antibodies with Rapid Novor we are able to pull out the CDR to graft them into our platform and test in vitro and in vivo.”
Getting Started with Surface Plasmon Resonance Services.
Requirements
- Antibody (100 μg, >90% purity, monoclonal)
- Antigen (at least 100 μg, >90% purity)
- Any standard buffer. Inquire about serum analysis.
*Ask us. If your samples don’t meet our requirements, please get in touch. We may be able to work with them.
Turn-Around Time
Rapid response to calls/emails.
Timing may be sample dependent.
Surface Plasmon Resonance Price.
Custom Kinetic Profile
- Suitable for detailed analysis of a few interactions
- Multi-cycle kinetics
- Optimized conditions per analysis
- Highly accurate Kon, Koff and KD profile
- Isoaffinity analysis
Ab-Ag Kinetic Profile
- Suitable for detailed analysis of a few antibodies
- Multi-cycle kinetics
- Optimized conditions per analysis
- Highly accurate Kon, Koff and KD profile
- Isoaffinity analysis
Antibody Screening by SPR
- Suitable for selecting the best antibodies from a larger set
- Standard conditions
- Single experiment
- Binding affinity and off rate screen
- Lead ranking
Epitope Binning by SPR
- Suitable for grouping antibodies by the epitope they bind
- Standard conditions
- Minimum 3×3 matrix
- Both orientations tested
*Per sample fees, setup and optimization costs depend on project requirements. Please inquire.
Talk to Our Scientists.
We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.
Talk to Our Scientists.
We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics
Talk to our scientists. We have sequenced over 9000+ antibodies and we are eager to help you.
Antibody Sequencing, Discovery and Characterization Services.
Antibody Sequencing & Discovery Services.
REmAb® mAb Sequencing
Monoclonal antibody sequencing from small antibody samples, no need for hybridoma or DNA information. Full sequence in record time.
Explore Antibody Sequencing Services
REpAb® pAb Sequencing
Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
Explore Antibody Discovery Services
RapidSPR™ Analysis
Label-free evaluation of antibody-antigen binding using surface plasmon resonance on Biacore or Nicoya. Can be employed for detailed kinetic profiling, kinetics screening, or epitope binning.
Explore SPR Services
RapidHDX-MS™
Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore HDX-MS Epitope Mapping Service
MATCHmAb™
Antibody verification by rapid peptide mapping. Antibody sequence confirmation and full detailed mapping for development purposes.
Explore Peptide Mapping Service
Antibody Characterization
LC-MS analysis for study of antibody glycosylation, disulfide bonds, post-translational modifications, sequence variants, intact mass and size.
Explore Antibody Characterization Services