Antibody Discovery Services.
REpAb is our mass spectrometry based in vivo antibody discovery service. A flexible end-to-end platform delivering developable, functional mAbs by starting with functional antibody proteins. Our antibody discovery service is mass spectrometry based, and powered by our unique pAb sequencing technology.
Our Antibody Discovery Platform.
How It Works.
Our unique technology allows in vivo antibody discovery by sequencing functional antibody proteins from serum, using mass spectrometry. We call this REpAb®. Leverage the entire natural immune response and begin the search for your lead molecule directly from functional native circulating antibodies.
You can plug our technology into your existing pipeline – for improved diversity and informed lead selection. Our antibody discovery platform can also stand alone, as a complete suite of services to take you from immunization and de novo polyclonal antibody (pAb) sequencing, through to recombinant mAb expression, and mAb characterization.
More Detail: Nature Communications
Le Bihan, T., Nunez de Villavicencio Diaz, T., Reitzel, C. et al. De novo protein sequencing of antibodies for identification of neutralizing antibodies in human plasma post SARS-CoV-2 vaccination. Nat Commun 15, 8790 (2024).
Species Agnostic Antibody Discovery Services
We can work with any species, including human antibodies and humanized animal. We are experienced in rabbit, alpaca, sheep, dog, chicken, human, and goat. Working with protein means you can test interaction with homologous targets in other model species early in your discovery campaign.
Immunization
We have standard immunization protocols ready to go, in several host species. Or we can coordinate your custom protocol with you and your chosen immunization team. A unique advantage of the REpAb® platform for tolerance busting is using different host species to raise antibodies against conserved proteins.
The REpAb® Advantage for Antibody Discovery.
What Antibodies Are You Missing?
Depending on when and where you sample B-Cells for genomic sequencing, you may miss the best antibodies, or they may be lost in the mix. We can find unique antibodies that are not present or not obvious in NGS data, which is crucial for therapeutic antibody discovery. If you’re not looking at the circulating antibody proteins, you’re missing out.
Broaden the Search.
Antibody discovery becomes easier when you:
- Search a bigger space and
- Improve the search strategy
Our REpAb® antibody discovery service can consider the entire immune response (up to 1018 sequences) and leverage biology to select the best antibodies.
Quality vs Quantity of Hits.
We start with the most abundant, functional, circulating antibodies from the host organism because they offer:
- Greater epitope diversity
- A wide range of binding affinities
- Reduced immunogenicity
At least 50% of REpAb® derived mAbs had desirable binding AND function (see Nature Communications paper, below).
Antibody Discovery Service Success Stories.
“Rapid Novor was tasked with finding highly specific, high affinity antibodies against an important target- and they delivered powerful antibodies fit-for-purpose. Such antibodies are not easily obtained yet the Rapid Novor approach proved immediately successful, despite our time-constrained and stringent demands.”
Get Started with Antibody Discovery.
Requirements
Antigen or antigen information. We’ve worked with peptides, proteins, enzymes, and small molecules.
Price
We offer fee-for-service for all projects.
Deliverables
- mAb sequences with paired HC and LC
- Optional: Recombinantly expressed mAbs
- Optional: SPR binding kinetics, screening and full profile
- Optional: HDX-MS epitope mapping report
Talk to Our Scientists.
We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.
Talk to Our Scientists.
We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics
Talk to our scientists. We have sequenced over 9000+ antibodies and we are eager to help you.
The Science of Antibody Discovery.
Antibody Sequencing & Discovery Services.
Antibody Sequencing, Discovery and Characterization Services.
Antibody Sequencing & Discovery Services.
REmAb® mAb Sequencing
Monoclonal antibody sequencing from small antibody samples, no need for hybridoma or DNA information. Full sequence in record time.
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REpAb® pAb Sequencing
Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
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RapidSPR™ Analysis
Label-free evaluation of antibody-antigen binding using surface plasmon resonance on Biacore or Nicoya. Can be employed for detailed kinetic profiling, kinetics screening, or epitope binning.
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RapidHDX-MS™
Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore HDX-MS Epitope Mapping Service
MATCHmAb™
Antibody verification by rapid peptide mapping. Antibody sequence confirmation and full detailed mapping for development purposes.
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Antibody Characterization
LC-MS analysis for study of antibody glycosylation, disulfide bonds, post-translational modifications, sequence variants, intact mass and size.
Explore Antibody Characterization Services