Protein Sequencing Service.
REmAb® is our de novo protein sequencing service, using mass spectrometry. REmAb has been unmatched in sequence accuracy, coverage and speed since 2015 thanks to continuous evolution of our protein sequencing technology. Our protein sequencing service determines the amino acid sequence of purified proteins with our proprietary chemistry and AI-powered bioinformatics algorithms.
How REmAb® De Novo Protein Sequencing Works.
The REmAb® de novo protein sequencing workflow delivers a complete protein sequence with unparalleled accuracy and reliability. Starting with just 100μg of purified protein, the sample undergoes multi-enzyme digestions and the fragments are measured with LC-MS/MS to derive the de novo peptide sequences. These fragments are then analyzed by Rapid Novor’s suite of bioinformatics algorithms to assemble the full-length protein sequence.
Our unique de novo workflow allows us to determine the full protein sequence with hundreds of overlapped peptides without requiring a database or sequence library for reference or homology.
The REmAb® Advantage.
| REmAb |
|---|
| Highest throughput: 50 to 100 mAbs per week |
| Highest accuracy: 100% |
| Lowest sample requirement: 50 μg |
| Most enzymes: 5-10 digests |
| Proprietary, next generation algorithms |
| Ile/Leu calls with WILD and WILDtag technology |
| Ability to handle the unexpected |
| Highest coverage: 100X |
| One sequence that works, every time |
| REmAb® | Others | |
|---|---|---|
| Throughput | 50 to 100 proteins per week | Single runs |
| Accuracy | 100% | Often low / ambiguous |
| Sample volume requirement | 100μg (as low as 7μg) | >150 μg |
| Multi-enzyme digest | At least 5 – 10 enzymes | 1 or 2 |
| Bioinformatics software | Proprietary, next generation algorithms | Previous generation commercial software |
| Accurate Ile/Leu Determination | Yes, WILD® and WILDtag technology | Rarely |
| Ability to handle the unexpected | Yes: additional chains, impurities, oligos, etc. | Rarely |
| Depth of coverage | 100X | Low or patchy |
| Downstream impact | One sequence that works, every time | Costly ambiguities to express and test |
WILD® Isoleucine-Leucine Determination.
Isobaric combinations with confidence: our patented WILD® method was the first commercial service to use additional peptide fragmentation via electron transfer high collision energy dissociation (EThcD) to create and analyze w-ions. This enables true Isoleucine-Leucine determination based on experimental evidence alone. Our unique and patented WILDtag chemistry increases coverage and confidence in isobaric assignments.
De Novo Sequencing From Any Species.
Our unique de novo workflow allows us to determine the full protein sequence without requiring a database or sequence library for reference or homology.
World Leading Experts In De Novo Protein Sequencing.
Our team comprises of experienced leaders in mass spectrometry proteomics, protein chemistry and bioinformatics. Even if your sample doesn’t meet our minimum requirements, come talk to us. REmAb® de novo protein sequencing is an important tool for scientists in sequence analysis.
Success Stories.
100% of respondents were happy with the experience
Respondents were 9.7/10 likely to refer their peers.
85% were already successful downstream. Remainder were in progress. No reported failure.
“You are certainly the best CRO we’ve ever worked with!❤️”
“We’ve focused on coupling cytokines with highly engineered VHVL of an antibody. So by sequencing the antibodies with Rapid Novor we are able to pull out the CDR to grant them into our platform and test in vitro and in vivo”
“Rapid Novor was tasked with finding highly specific, high affinity antibodies against an important target – and they delivered powerful antibodies fit-for-purpose. Such antibodies are not easily obtained yet Rapid Novor approach proved immediately successful, despite our time-constrained and stringent demands”
Getting Started.
Requirements.
- >100 μg of protein
- >80% purity
Is your protein sample smaller or less pure?Ask us. We’ve sequenced many proteins when the sample amount was much less than our requirements.
Deliverables.
- Complete protein sequence in FASTA
- Interactive peptide coverage viewer and report
- Notable observations
Turn-Around Time.
De Novo Protein Sequencing Pricing.
Intro Price
- For any number of proteins in the first order.
- * Price dependent on protein size, inquire to find out more.
If your protein is an Antibody, please refer to the pricing for Antibody Sequencing.
Optional Add Ons.
Talk to Our Scientists.
We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.
Talk to Our Scientists.
We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics
Talk to our scientists. We have sequenced over 9000+ antibodies and we are eager to help you.
The Science of Protein Sequencing.
REmAb® De Novo Protein Sequencing Applications.
Facilitate in silico design, rational engineering, humanization, chimerization, affinity maturation, conjugation design and construction of fragments and multi-specifics.
Identify novel targets for therapy or diagnosis. De novo sequence and generate recombinant antibodies for downstream binding affinity or specificity enhancement.
Antibody Sequencing, Discovery and Characterization Services.
Antibody Sequencing & Discovery Services.
REmAb® mAb Sequencing
Monoclonal antibody sequencing from small antibodysamples, no need for hybridoma or DNA information. Full sequence in record time.
Explore Antibody Sequencing Services
REpAb® pAb Sequencing
Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
Explore Polyclonal Antibody Sequencing
MATCHmAb™
Antibody verification by rapid peptide mapping. Antibody sequence confirmation and full detailed mapping for development purposes.
Explore Peptide Mapping
RapidHDX-MS™
Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore HDX-MS Epitope Mapping
RapidSPR™ Analysis
Label-free evaluation of antibody-antigen binding using surface plasmon resonance on Biacore or Nicoya. Can be employed for detailed kinetic profiling, kinetics screening, or epitope binning.
Explore SPR Analysis
Antibody Characterization
LC-MS analysis for study of antibody glycosylation, disulfide bonds, post-translational modifications, sequence variants, intact mass and size.
Explore antibody characterization