Epitope Mapping Service via HDX-MS.
RapidHDX-MS™ is our mass spectrometry based epitope mapping service, that can identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution. Our HDX MS service can study complex binding, and identify linear and conformational epitopes. Integrated AI enables us to scale up to 100’s of mAbs at a time.
How our epitope mapping services work.
The HDX-MS Epitope Mapping Workflow
Hydrogen-Deuterium-eXchange Mass Spectrometry (HDX-MS) measures the exposure levels of amide hydrogen atoms on the surface of a protein or protein complex. The first step causes the exposed hydrogen atoms to be readily exchanged with deuterium. Hydrogen atoms that are buried within the protein structure – for instance at the binding site of an antibody-antigen complex – are not exchanged as readily. By examining the peptides using bottom-up mass spectrometry, and comparing them with the amino acid sequence, it is then possible to determine which regions are the binding locations between the interacting partners.
- Labeling – incubate different fractions with D2O solution in a time series
- Quench – chill and control temperature to prevent further exchange or back-exchange
- Digest – prepare peptides for analysis
- LC-MS/MS
- Data analysis- determine deuterium uptake of digested peptides acid and identify binding site(s)
The AI + HDX-MS Epitope Screening Workflow
In epitope screening analyses, 100’s of antibodies are first sorted into bins based on their predicted epitopes using AI. Subsequently, HDX-MS experiments confirm epitope predictions and increase resolution. These experimentally determined epitope maps are fed back to the AI to refine the epitope predictions for related antibodies. In single antibody-antigen analyses, AI generated epitope predictions can guide experimenters to more detailed study of certain regions, or simply increase confidence in the results.
Advantages of Our HDX-MS Epitope Mapping Services.
Higher resolution epitopes.
Instrumentation matters.
Ion Mobility mass spectrometry is required for successful HDX-MS experiments – it allows for separate measurements of peptides of the deuterated and non-deuterated peptides that co-elute from even the best liquid chromatography columns. Robotic sample preparation is required for repeated analysis under the same conditions. Our Waters Cyclic IMS mass spectrometer and Trajan PAL HDX sample handler are the best-in-class instruments, providing the best possible HDX-MS results.
Epitope mapping service packages.
HDX-MS Epitope Mapping
- Detailed epitope mapping analysis for 1 antibody-antigen pair.
- Requirements: Antigen 200 μg, Antibody 200 μg, Antigen sequence
- Add ons: AI predictions, Paratope mapping, additional mAbs
- Turn around time: 3-4 weeks
HDX-MS + AI Epitope Screening
- Epitope mapping for 100’s of antibodies against one antigen.
- Requirements: Antigen 500 μg, Antibodies 100 μg each, Antibody sequences, Antigen 3D structure
- Add ons: additional antibodies
- Turn around time: 4-6 weeks
Customized HDX-MS Projects
- Custom analysis of protein complexes, conformation changes, ligands and other structural questions.
- Please inquire about experiment design, and sample requirements.
Add-on and related services
Getting Started.
Talk to Our Scientists.
We Have Sequenced 10,000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.
Talk to Our Scientists.
We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics
Talk to our scientists. We have sequenced over 9000+ antibodies and we are eager to help you.
The Science of Epitope Mapping.
Antibody Sequencing & Discovery Services.
Antibody Sequencing, Discovery and Characterization Services.
Antibody Sequencing & Discovery Services.
REmAb® mAb Sequencing
Monoclonal antibody sequencing from small antibodysamples, no need for hybridoma or DNA information. Full sequence in record time.
Explore Antibody Sequencing Services
REmAb® Protein Sequencing
Monoclonal antibody sequencing from small antibodysamples, no need for hybridoma or DNA information. Full sequence in record time.
Explore Antibody Sequencing Services
REpAb® pAb Sequencing
Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
Explore Polyclonal Antibody Sequencing
MATCHmAb™
Antibody verification by rapid peptide mapping. Antibody sequence confirmation and full detailed mapping for development purposes.
Explore Peptide Mapping
RapidSPR™ Analysis
Label-free evaluation of antibody-antigen binding using surface plasmon resonance on Biacore or Nicoya. Can be employed for detailed kinetic profiling, kinetics screening, or epitope binning.
Explore SPR Analysis
Antibody Characterization
LC-MS analysis for study of antibody glycosylation, disulfide bonds, post-translational modifications, sequence variants, intact mass and size.
Explore antibody characterization